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Title: Calcifying bacteria mediated cementation for improvement of building materials
Researcher: Dhami, Navdeep Kaur
Guide(s): Reddy, M. Sudhakara and Mukherjee, Abhijit
Keywords: Bacterial carbonic anhydrase
Bacterial urease
Engineering and Technology
Microbially induced calcium carbonate precipitation
University: Thapar Institute of Engineering and Technology
Completed Date: 2013
Abstract: Microbially induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process in which microbes produce inorganic materials as part of their basic metabolic activities. The hydrolysis of urea by microbial urease has been found to generate carbonate ions without an associated production of protons. In the presence of calcium rich environment, calcium carbonate crystals are produced which form solid crystalline material. The evidence of ureolytic bacterial involvement in precipitation of carbonates is a new revolution in the field of industrial microbiology. The application of these bacteria for production of calcium carbonates has recently emerged as a method for protecting and consolidating decayed construction materials as carbonate crystals precipitated are highly coherent and durable. The objective of the present study was to explore calcifying bacterial diversity from alkaline soils, understand the actual function and role of bacteria in carbonate precipitation, investigate the survival of bacterial cells within the building materials and finally develop a technology for enhancing the durability of energy efficient, low cost building materials. Ubiquitous nature of bacteria makes them ideal candidates for isolating them from extreme environments. The bacteria were isolated from calcareous soils so that they have the ability to survive in alkaline environment of various building materials. In the present work, five ureolytic and calcifying bacteria were isolated and characterized for production of various polymorphs of calcium carbonates. Role of enzymes urease and carbonic anhydrase in calcium carbonate precipitation were investigated and most efficient calcifying bacteria Bacillus megaterium SS3 was employed to improve the durability of various energy efficient building materials and building materials produced from industrial by products.
Pagination: xxxv, 341p.
Appears in Departments:Department of Biotechnology

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