Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/22107
Title: Glycogenome Reannotation and identification of novel glycoproteins from mycobacterium tuberculosis H37rv
Researcher: Lakshmanan, J
Guide(s): Rameshkumar, G
Keywords: Glycogenome
Glycoproteins
Mycobacterium
Tuberculosis H37rv
Upload Date: 5-Aug-2014
University: Anna University
Completed Date: n.d.
Abstract: Mycobacterium tuberculosis causative agent of human tuberculosis newline TB was responsible for nearly 1 5 million deaths annually accounting newlinealmost 2 4 deaths worldwide The co infection with HIV the evolution of newlinemulti drug resistance MDR extreme drug resistance XDR and more newlinerecently totally drug resistance TDR strains severely hamper the newlineanti tuberculosis therapy The Cell wall associated and secreted newlineglycoconjugates including Lipoarabinomannan LAM Lipomannan LM newlineArabinogalactans AG Phosphoinosidemannoside PIM Cord factor newlineCapsular glucans and Culture filtrate glycoproteins plays an important role in newlinevirulence and pathogenesis of M tuberculosis The current understanding of newlineenzymes involved in the biosynthesis of these functionally important newlineglycoconjugates termed as carbohydrate glycan modifying enzymes newline glycogenome or glycogenes was not sufficient due to lack apparent function newlineassigned to nearly 40 of the coding sequences in M tuberculosis genome newlineHere we report the use of comprehensive Bioinformatics workflow for newlinereannotation of all unknown function proteins from M tuberculosis H37Rv newlinegenome Our analysis predicted 106 novel putative glycan modifying genes newlineFurther non homology based gene cluster analysis was used to predict newlineputative N Glycosylation pathway newlineApart from all the above glycoconjugates M tuberculosis might newlinealso employs several cell wall glycoproteins whose role in virulence and newlinepathogenesis was poorly understood The specific carbohydrate binding newlinelectins immobilized on agarose beads was used to capture cell wall newlineglycoproteins Glyco catch The results showed M tuberculosis cell wall newlinecontains several glycoproteins with terminal mannose ConA PSA and LCU newlineand N Acetylglucosamine DSA Two prominent protein bands eluted from newlinemannose specific ConA lectin was analyzed further using MALDI TOF and newlineESI MS methods The two proteins were identified as hypothetical protein newline 23 KDa and transferase 32
Pagination: xvi, 196p.
URI: http://hdl.handle.net/10603/22107
Appears in Departments:Faculty of Science and Humanities

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01_title.pdfAttached File167.6 kBAdobe PDFView/Open
02_certificate.pdf5.56 MBAdobe PDFView/Open
03_abstract.pdf77.27 kBAdobe PDFView/Open
04_acknowledgement.pdf62.74 kBAdobe PDFView/Open
05_contents.pdf139.93 kBAdobe PDFView/Open
06_chapter 1.pdf1.2 MBAdobe PDFView/Open
07_chapter 2.pdf393.33 kBAdobe PDFView/Open
08_chapter 3.pdf4.35 MBAdobe PDFView/Open
09_chapter 4.pdf5.35 MBAdobe PDFView/Open
10_chapter 5.pdf1.62 MBAdobe PDFView/Open
11_chapter 6.pdf88.94 kBAdobe PDFView/Open
12_appendix.pdf297.42 kBAdobe PDFView/Open
13_references.pdf284.05 kBAdobe PDFView/Open
14_publications.pdf83.88 kBAdobe PDFView/Open
15_vitae.pdf72.3 kBAdobe PDFView/Open


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