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Title: Identify and correlate the pathogenic bacteria in dental unit water and dental unit waterline biofilm and its eradication
Researcher: Irfana Fathima, S.
Guide(s): Kandaswamy, D.
Keywords: Biofilm formation
Metagenomic analysis
Negative bacteria
Pathogenic bacteria
Ultrasonic scaler
University: Sri Ramachandra University
Completed Date: 14/08/2018
Abstract: Dental unit waterlines are narrow plastic tube which carries water to the high speed hand piece the air/water syringe the ultrasonic scaler The narrow plastic tube favours biofilm formation which tends to contaminate the Dental unit water used for treating patients This study was undertaken to identify the presence of bacteria in the Dental unit waterline biofilm using Real time polymerase chain reaction and to identify and correlate the bacterial load in the DUWL biofilm and the DUW using Next generation sequencing technique NGS and to eradicate biofilm from DUWLs Institutional Ethics Committee approval was sought prior to the start of the study which was conducted in three phases Conservative and Endodontic procedures were performed Phase I is the preliminary study in which the presence of bacteria in the DUWL biofilm and the formation of biofilm in the inner lumen of the DUWLs were analyzed The presence of bacteria in the DUWL was assessed in ten DUs One new polyurethane tube served as a control The tubes were in use for a week and the biofilm sample was extracted from the inner lumen of the DUWL by scraping technique. The extracted biofilm samples was analyzed for Gram positive bacteria GPB and Gram negative bacteria GNB targeting 16S rRNA in Qpcr Formation of biofilm in the inner lumen of the DUWLs was analyzed at different days with a time interval using scanning electron microscopy The tubes were removed from the DUs at different intervals 7th day 10th day 13th day and 16th day The tubes were split lengthwise using a sterile single edge blade B.P blade and the biofilms in the inner lumen of the tube were analyzed for biofilm formation In phase II pathogenic bacteria in the DUWLs biofilm were identified and correlated with the DUW Thirteen DUs were labelled in the study twelve DUs were used for dental procedure and one DU was not used for any dental procedure In addition water was allowed to be stagnant in the DUWL After 10 days DUWL biofilm was extracted and DUW was collected from thirteen DUs and analyzed
Pagination: 1 75
Appears in Departments:Dental College

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