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Title: Phylogenetic diversity of medically important fusarium species using multi locus sequence typing and their in vitro susceptibility profiles to conventional and newer antifungals
Researcher: Ananya T.S.
Guide(s): Anupma Jyoti Kindo
Keywords: Antifungals
Clinical isolates
Fusarium infection
Pathogenic species
University: Sri Ramachandra University
Completed Date: 04/08/2018
Abstract: Fusarium species cause a broad spectrum of infections in humans, including superficial locally invasive and disseminated infections Risk factors for severe fusariosis include prolonged neutropenia and T cell immunodeficiency especially in hematopoietic stem cell transplant recipients with severe graft versus host disease The most frequent presentation of disseminated fusariosis is a combination of characteristic cutaneous lesions and positive blood cultures with or without lung or sinus involvement The prognosis is poor and is determined largely by degree of immunosuppression and extent of infection with virtually a 100 per cent death rate among persistently neutropenic patients with disseminated disease This study was conducted to know about human Fusarium infections in and around Tamil Nadu India to learn about the most commonly occurring pathogenic species their phylogenetic diversity with evolutionary relationships and their susceptibility profiles to conventional and newer antifungal including ravuconazole and efinaconazole 125 human cases of Fusarium infections were studied and 108 clinical isolates were identified up to species level by DNA sequencing 83 isolates were included for Multi Locus Sequence Typing MLST and were tested against eight antifungal For the MLST study four target loci were selected namely the Translation Elongation Factor Tubulin Internal Transcribed Spacer and the second largest subunit of DNA dependent RNA Polymerase II gene Molecular identification was performed using comparative sequence analysis with three online databases Fusarium ID Fusarium MLST and NCBI GenBank To aid unambiguous molecular identification of Fusarium, the sequences were deposited in the NCBI GenBank database Phylogenetic analysis was performed with concatenated sequences of the four target loci using MEGA7 The eight antifungal were tested using the CLSI M38 A2 broth micro dilution method namely amphotericin B voriconazole posaconazole ravuconazole efinaconazole caspofungin natamycin and terbinafine Fusarium sol
Pagination: 1 138
Appears in Departments:Medical College

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