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Title: Studies on the induction of variation through in vitro culture in Jatropha curcas L.
Researcher: Jose, Jikku
Guide(s): Nambisan, Padma
Keywords: Biotechnology
Jatropha curcas L.
Upload Date: 19-Jun-2014
University: Cochin University of Science and Technology
Completed Date: 30/1/2013
Abstract: Given the economic importance of Jatropha curcas, and its limited availability in the wild, it would be desirable to establish plantations ofthe tree so as to obtain assured supply of raw material for extraction of phytochemicals, and seeds for production of biodiesel. However both seed propagation as well as propagation by cuttings is unsatisfactory in this tree species. Seeds have poor viability and are genetically heterozygous leading to genetic variability in terms of growth, biomass, seed yield, and oil content. Stern cuttings have poor roots and the trees are easily uprooted. Tissue culture techniques could possibly be gainfully employed in the propagation of elite plants ofJaIropha. When plant tissue is passaged through in vitro culture, there is possibility of induction of variations. An estimation of somaclonal variability is useful in a determination of culture protocols. Molecular markers could be employed to estimate the amount of variations induced in callus and regenerants by different honnonal combinations used in culture. In this context the present study aims to develop an in vitro propagation protocol for the production of plantlets and to evaluate the variation induced in callus and regenerants in comparison with mother plant by the use of molecular markers and by studying phytochemicals and bio active compounds present in callus and regenerated plants
Pagination: 236p.
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File40.02 kBAdobe PDFView/Open
02_dedication.pdf151.63 kBAdobe PDFView/Open
03_certificate.pdf42.42 kBAdobe PDFView/Open
04_declaration.pdf39.14 kBAdobe PDFView/Open
05_acknowledgement.pdf74.84 kBAdobe PDFView/Open
06_abbreviations.pdf52.78 kBAdobe PDFView/Open
07_contents.pdf122.46 kBAdobe PDFView/Open
08_chapter 1.pdf173.11 kBAdobe PDFView/Open
09_chapter 2.pdf5.6 MBAdobe PDFView/Open
10_chapter 3.pdf3.09 MBAdobe PDFView/Open
11_chapter 4.pdf1.93 MBAdobe PDFView/Open
12_chapter 5.pdf54.8 kBAdobe PDFView/Open
13_appendix.pdf149.46 kBAdobe PDFView/Open
14_references.pdf513.22 kBAdobe PDFView/Open

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