Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/176076
Title: quotALTERATION IN MITOCHONDRIAL GENE EXPRESSION AND ACTIVATING INTRINSIC APOPTOTIC PATHWAY IN HUMAN CERVICAL CANCER CELL BY PROOXIDANT ISOLATED FROM BARK OF SYMPLOCOS COCHINCHINENSIS LOUR MOOREquot
Researcher: Rajesh Kumar, R
Guide(s): Dhanbal , S.P.
Keywords: Symplocos cochinchinensis, HeLa Cancer cells, Cytotoxicity, Gene expression, intrinsic apoptotic pathway, Bcl2, Bax
University: JSS University
Completed Date: 2016
Abstract: quot newlineThe objective of the present investigation was to isolate active constituents from the fruits of Elaeagnus latifolia, bark of Rhododendron arboretum ssp. nilagiricum (Zenker) Tagg.J.B. Stevenson and bark of Symplocos cochinchinensis and evaluate for their anticancer property by altering the gene expression in mitochondria and inducing intrinsic apoptosis in HeLa human cervical cancer cell. newline newlineFruits of Elaeagnus latifolia, bark of Rhododendron arboretum ssp. nilagiricum (Zenker) Tagg.J.B. Stevenson and bark of Symplocos cochinchinensis were selected for the study. Hot continuous extraction was carried out for all the plants and yielded 12 extracts. The potent extracts were selected based on their antioxidant, prooxidant and cytotoxicity property. Two extracts were selected for isolation of active compound. After characterization, one compound was selected for induction of apoptosis in HeLa cells using various apoptosis induction method. newline newlineAntioxidant, prooxidant and cytotoxicity study was carried out for all the extracts. Ethyl acetate and 50% methanolic extracts of Symplocos cochinchinensis showed potent activity in all the methods. Hence these potent extracts were selected for isolation of active compound. Isolation yielded 5 compound from both extracts. Further FTIR, 13CNMR, 1HNMR and LC-MS analysis of all 5 compounds led to identification of three known compound such as Ellagic acid, Odoroside, Dammaranediol and two new compounds RD1, RD2. Then cytotoxicity was carried out for RD1 and RD2. Based on the potent cytotoxicity RD2 was selected for in vitro studies on induction of apoptosis and apoptotic DNA fragmentation, changes in mitochondrial membrane potential, and profiles of gene and protein expression in response to treatment of HeLa cells. RD2 exhibited potential apoptotic effects on HeLa cells, and they induced apoptosis in these cells through the events of up regulation of caspase 3, downregulation of bcl-2 family genes followed by cytochrome c release. newlinequot
Pagination: I-XV, 1-106p
URI: http://hdl.handle.net/10603/176076
Appears in Departments:College of Pharmacy

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02_certificates.pdf1.7 MBAdobe PDFView/Open
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04_contents.pdf82.13 kBAdobe PDFView/Open
05_abstratct.pdf149.76 kBAdobe PDFView/Open
06_list of tables and figures.pdf82.18 kBAdobe PDFView/Open
07_chapter 1.pdf319.73 kBAdobe PDFView/Open
08_chapter 2.pdf160.27 kBAdobe PDFView/Open
09_chapter 3.pdf157.92 kBAdobe PDFView/Open
10_chapter 4.pdf259.24 kBAdobe PDFView/Open
11_chapter 5.pdf738.19 kBAdobe PDFView/Open
12_chapter 6.pdf2.13 MBAdobe PDFView/Open
13_chapter 7.pdf160.05 kBAdobe PDFView/Open
14_chapter 8.pdf83.6 kBAdobe PDFView/Open
15_references.pdf269.14 kBAdobe PDFView/Open


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