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Title: Synthesis and membrane association of hydrophobic peptides with covalently linked fatty acids
Researcher: Joseph, Mercy
Guide(s): Nagaraj, R
Keywords: Cellular and molecular biology
hydrophobic peptides
fatty acids
Upload Date: 3-Mar-2014
University: Jawaharlal Nehru University
Completed Date: 1994
Abstract: Proteins undergo various modifications during or after translation. Several proteins of newlineprokaryotic, eukaryotic viral origins have been found to be modified with covalently linked newlinefatty acids and very recently modification with isoprene units have also been observed. newlineThere is extensive documentation about the proteins that are acylated, the sequences in the newlineregion o.f acylation and the enzymes that are involved in this modification. Most of the newlineproteins that are acylated perform important functions like regulating cell growth and these newlinemodification are required for their optimal functioning. However, there has not been much newlineinformation as to how covalently linked fatty acid might modulate the association of the newlinepeptide chain with membranes. Hence, work directed towards this was initiated. Synthetic newlinepeptides corresponding to the acylation regions in proteins and their fatty acyl derivatives newlinewere synthesized. Their conformations and interactions with membranes were investigated. newlineThe contents of this thesis are divided into six chapters and are as follows:- newlineChapter 1 : A brief description about the diverse group of structurally and functionally newlinedistinct proteins that are acylated, the various aspects of fatty acid acylation, the enzymes newlinethat are involved in these modifications and how these modifications are necessary for newlineoptimal functioning of these proteins are reviewed. newlineChapter 2 : The source of chemicals, purification of solvents, preparation of amino acid and newlinefatty acid derivatives, experimental protocols followed in solution and solid phase peptide newlinesynthesis, details of amino acid and fatty acid analysis and purification of lipids are newlinedescribed in this chapter. newlineIf newlineChapter 3 : This chapter deals with various protocols involved in the synthesis of peptides newlineand their fatty acyl derivatives. The general procedures followed and the details of newlineindividual peptides obtained by solution phase and segment condensation methods are newlinedescribed.
Pagination: iv, 89p.
Appears in Departments:Centre for Cellular and Molecular Biology

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01_title.pdfAttached File19.07 kBAdobe PDFView/Open
02_certificate.pdf18.53 kBAdobe PDFView/Open
03_acknowledgements.pdf47.38 kBAdobe PDFView/Open
04_contents.pdf50.24 kBAdobe PDFView/Open
05_abbrevations.pdf57.36 kBAdobe PDFView/Open
06_abstract.pdf119.19 kBAdobe PDFView/Open
07_list of publications.pdf26.84 kBAdobe PDFView/Open
08_chapter 1.pdf696.18 kBAdobe PDFView/Open
09_chapter 2.pdf337.93 kBAdobe PDFView/Open
10_chapter 3.pdf670.11 kBAdobe PDFView/Open
11_chapter 4.pdf529.37 kBAdobe PDFView/Open
12_chapter 5.pdf2.17 MBAdobe PDFView/Open
13_chapter 6.pdf139.34 kBAdobe PDFView/Open
14_references.pdf922.03 kBAdobe PDFView/Open

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