Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/16627
Title: Molecular studies on the role of cell-fibronectin interaction in tumourigenicity
Researcher: Bhattacharya, Amit Kumar
Guide(s): Pande, Gopal
Keywords: Molecular Biology
Cellular Biology
purification
polyma
RNA isolation
immumoprecipitation
Upload Date: 3-Mar-2014
University: Jawaharlal Nehru University
Completed Date: 1994
Abstract: It is now well established that the interaction of cells with molecules of the newlineextracellular matrix can regulate various cell functions. Specifically, the newlineinteraction of cells with fibronectin (FN), plays an important role in this regard, newlineand therefore its regulation is of physiological significance. In principle, ceii-FN newlineinteractions can be regulated either (1) by altering levels of FN binding integrin newlinereceptors on the cell surface, or (2) by post translational modifications of the newlineintegrins involved. fn the present investigation, the mechanisms regulating the newlineinteraction of FN with the a5~ 1 integrin have been studied and their significance newlinein determining tumourigenic abilities of transformed cells and morphological newlinecharacteristics of normal mitotic cells has been examined. For this purpose three newlinecell systems have been used: (1) AK-5 tumour (2) polyoma virus transformed newlinefibroblasts (3) mitotic and interph.ase fibroblasts. newlineAK-5: a histiocytoma newlineAK-5 is an ascitic histiocytoma, consisting of four subpopulations (Band I - newlineBand IV cells) based on their bouyant density. The two major subpopulations newlinediffer markedly- Band I cells which are the lightest are more tumourigenic and newlinehave higher levels of cell surface FN than Band IV cells, which are the heaviest. newlineBand II and Ill cells are minor subpopulations with intermediate characteristics newline(Exp. Cell Res. 17 4, 41-48, 1988). In this study it has been found that Band I newlinecells have higher attachment ability to substrates coated with FN (4.0 times newlinemore) than Band IV cells. The differential ability to interact with FN is at least newlinepartly due to the higher levels (1.5 times more) of a 5 ~ 1 integrin on the cell newlinesurface of Band I cells than Band IV cells, as determined by flow cytometric newlineanalysis. The steady state mANA levels of the a 5 and ~ 1 subunits are also newlinecorrespondingly higher in Band I and Band IV cells. newline
Pagination: iv, 66p.
URI: http://hdl.handle.net/10603/16627
Appears in Departments:Centre for Cellular and Molecular Biology

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02_certificate.pdf19.63 kBAdobe PDFView/Open
03_acknowledgements.pdf61.04 kBAdobe PDFView/Open
04_contents.pdf90.63 kBAdobe PDFView/Open
05_abbrevations.pdf43.81 kBAdobe PDFView/Open
06_abstract.pdf143.37 kBAdobe PDFView/Open
07_chapter 1.pdf652.71 kBAdobe PDFView/Open
08_chapter 2.pdf708.61 kBAdobe PDFView/Open
09_chapter 3.pdf4.97 MBAdobe PDFView/Open
10_chapter 4.pdf494.47 kBAdobe PDFView/Open
11_references.pdf348.33 kBAdobe PDFView/Open


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