Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/16188
Title: Isolation and molecular characterization of Escherichia coli O157 H7 and other pathogenic E coli from Dairy herds feaces and wastewater of Solan District of Himachal Pradesh
Researcher: Sharma, Rakhi
Guide(s): Soni, N.K
Keywords: Dairy
E. coli
Flora
Gastrointestinal
Pathogen
Waste Water
Upload Date: 24-Feb-2014
University: Shoolini University of Biotechnology and Management Sciences
Completed Date: 11/10/2012
Abstract: newline newline ABSTRACT newline Escherichia coli is the predominant facultative anaerobe of the human colonic flora. The organism typically colonizes the infant gastrointestinal tract within hours of life. In present study, 50 feacal samples were collected from various herd s at different places in and around Solan, thus all collected samples were processed for isolation and biochemical identification. Out of 50, 36 (72%) found positive for E.coli as discussed under Table(4.2). All isolates were antigenically characterised for O antigen Table(4.3) and among 28, 8 (22%) isolates were untypable, 5 (13.8%) were O153, 5(13.8%) were O11, 3(8.3%) O60, 3(8.3%) O90, 3(8.3%) O91, 2(5.5%) O147, 2(5.5%) O4, 2(5.5%) O6, 1(2.7%) O35, 1(2.7%) O135, 1(2.7%) O106; All isolates were subjected to polymerase chain reaction for the presence of stx1,stx2, eaeA, hlyA genes among strains of E.coli isolated from Dairy Herd s feaces. Among 36 samples 4(11%) samples were found positive for the presence of eaeA gene while on among all 1(2.7%) found positive for stx2 gene. newline
Pagination: xii, 70 p.
URI: http://hdl.handle.net/10603/16188
Appears in Departments:Faculty Of Biotechnology

Files in This Item:
File Description SizeFormat 
10_abbreviations.pdfAttached File7.51 kBAdobe PDFView/Open
11_chapter1.pdf337.5 kBAdobe PDFView/Open
12_chapter2.pdf287.17 kBAdobe PDFView/Open
13_chapter3.pdf324.55 kBAdobe PDFView/Open
14_chapter4.pdf916.63 kBAdobe PDFView/Open
15_chapter5.pdf179.44 kBAdobe PDFView/Open
16_bibliography.pdf317.34 kBAdobe PDFView/Open
17_appendix i.pdf275.32 kBAdobe PDFView/Open
1_title.pdf88.83 kBAdobe PDFView/Open
2_bonafidecertificate.pdf170.47 kBAdobe PDFView/Open
3_declaration.pdf243.05 kBAdobe PDFView/Open
4_certificate.pdf243.42 kBAdobe PDFView/Open
5_abstract.pdf162.13 kBAdobe PDFView/Open
6_acknowledgement.pdf111.46 kBAdobe PDFView/Open
7_tableofcontent.pdf90.35 kBAdobe PDFView/Open
8_list of tables.pdf82.05 kBAdobe PDFView/Open
9_listoffigures.pdf86.64 kBAdobe PDFView/Open


Items in Shodhganga are protected by copyright, with all rights reserved, unless otherwise indicated.