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Title: Assessment of anti inflammatory potential and characterisation of bioactive fractions from Gentiana Kurroo Royle and Artemisia amygdalina Decne
Researcher: Qayoom, K. Mubashir
Guide(s): Ganai, B. A. and Seema Akbar
Keywords: Artemisia amygdalina Decne, Gentiana kurroo Royle
Medicinal plants, Anti-inflammatory potential.
University: University of Kashmir
Completed Date: 2015
Abstract: Artemisia amygdalina Decne and Gentiana kurroo Royle are important medicinal plants belonging to two different plant families. These two plants were worked out for the evaluation of their anti-inflammatory potential. Carrageenin induced paw edema model was used for screening of different extracts of these plants against acute inflammation. In both plants methanolic extract showed maximum potential in inhibition of paw edema. These extracts were also studied in humoral and cellular immune models, where again, the most bioactive fraction found was methanolic extract in both plants. So methanolic extracts were studied in detail for dose dependent effect in acute and chronic inflammation in vivo. Humoral and cellular immune models were used for observing the effect of methanolic extracts from both plants against the humoral and cell mediated immunity. In vitro study was also carried out using LPS stimulated peritoneal macrophages and splenocytes isolated from immunized Balb/C mice. These cells were cultured in presence of different concentrations of methanolic extracts and taking betamethasone as standard drug. The supernatants obtained from the culture of LPS stimulated macrophages were analysed for TNF-and#945;, NO and IL-6 concentrations. NF-and#1082;B was quantified from the pelleted macrophages by western blotting using anti- NF-and#1082;B antibodies. These methanolic extracts were further characterised using LC-ESI-MS/MS. The results obtained from these studies showed anti-inflammatory potential in methanolic extract of these plants. These extracts decreased edema formation in both acute and chronic inflammation in a dose dependent manner. Both the humoral and cell mediated immune responses were suppressed in extract treated animals in vivo. The immune suppression by these extracts was further seen by checking serum TNF-and#945; level in animals of these immune models. Again the extract treated animals had decreased serum TNF-and#945; level as compared to untreated animals.The results from in vitro study showed inhibitory potential of methanolic..
Appears in Departments:Department of Biochemistry

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01_title.pdfAttached File174.57 kBAdobe PDFView/Open
02_certificate.pdf78.5 kBAdobe PDFView/Open
03_declaration.pdf77.15 kBAdobe PDFView/Open
04_acknowledgement.pdf37.22 kBAdobe PDFView/Open
05_contents.pdf147.6 kBAdobe PDFView/Open
06_list_of_tables_and_figures.pdf152.72 kBAdobe PDFView/Open
07_abbreviations.pdf141.58 kBAdobe PDFView/Open
08_abstract.pdf138.23 kBAdobe PDFView/Open
09_introduction.pdf260.94 kBAdobe PDFView/Open
10_review of literature.pdf411.17 kBAdobe PDFView/Open
11_methodolgy.pdf474.2 kBAdobe PDFView/Open
12_results.pdf1.38 MBAdobe PDFView/Open
13_discussion.pdf479.65 kBAdobe PDFView/Open
14_conclusion.pdf333.44 kBAdobe PDFView/Open
15_references.pdf614.61 kBAdobe PDFView/Open

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