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Title: Tissue culture studies on artemisia vulgaris L
Researcher: Rezvan Karami Borzabad
Guide(s): Sudarshana, M S
Keywords: Botany
Tissue culture studies
artemisia vulgaris
Upload Date: 17-Feb-2014
University: University of Mysore
Completed Date: August 2010
Abstract: The present in vitro studies was carried out on A. vulgaris L. A medicinal newlineherb. It was observed that MS medium elicited a better response than B-5 and newlineWhite ,s media to be suitable for supporting morphogenic potency of the leaf and newlinestem explants. Luxuriant embryogenic callus was observed on MS medium newlinecontaining (1.0 mgl-1) BAP and (3.0 mgl-1) GA3.Callus from MS+BAP (0.5 mgl-1) newline+ 2,4-D (0.5-1.5 mgl-1) + ascorbic acid(40-120 mgl-1) was subcultured on newlineMS+BAP (1.0mgl-1) + GA3(3.0mgl-1) + IAA (0.5 mgl-1) produced embryogenic newlinecallus with shoot buds and roots. On IAA supplemented medium roots were newlineobserved from the base of micro shoots within 20 days of transfer. After 3-4 newlineweeks of incubation, the well grown shoots having well developed roots were newlineseparated and transferred to pots for hardening. The survival rate was 68%, the newlineregenerated plantlets were phenol typically similar when compared with in vivo newlineplant. Histological studies were made at different stages of development starting newlinefrom the initiation of the callus, up to the induction of embryoid and shoot buds. newlineHistological studies of leaf calli revealed that shoot and root developed from newlinemeristematic centres depends upon the nature of growth regulator used. Somatic newlineembryos developed only from nodular embryogenic tissues and never from newlinedisorganized callus. Microscopic observations of suspensions after 10 days newlineexhibited single cells with various shapes and sizes. Upon transfer to basal newlinesuspension medium embryogenic cells was observed.The cell suspension newlinecontained free cells, cell aggregates, xylem elements and vacuolated cells and a newlinefew organized ones. Five-week-old clusters became nodular and somatic embryos newlineat different stages of development with different shapes with suspensor were newlinevisible. Cells stained with 0.1% haematoxyline showed more than 95% newlineviability.The formation of bipolar adventive embryos begins just as in newlineorganogenesis with the dedifferentiation of the isolated tissue or cells but proceeds newlinesubsequently in a different way
Pagination: 163p.
Appears in Departments:Department of Botany

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01_title.pdfAttached File33.88 kBAdobe PDFView/Open
02_dedication.pdf32.57 kBAdobe PDFView/Open
03_certificate.pdf63.1 kBAdobe PDFView/Open
04_abstract.pdf25.63 kBAdobe PDFView/Open
05_acknowledgement.pdf35.26 kBAdobe PDFView/Open
06_abbreviations.pdf30.46 kBAdobe PDFView/Open
07_contents.pdf31.26 kBAdobe PDFView/Open
08_list of tables.pdf31.66 kBAdobe PDFView/Open
09_list of figures.pdf64.93 kBAdobe PDFView/Open
10_list of graphs.pdf30.55 kBAdobe PDFView/Open
11_chapter 1.pdf87.89 kBAdobe PDFView/Open
12_chapter 2.pdf4.37 MBAdobe PDFView/Open
13_chapter 3.pdf4.3 MBAdobe PDFView/Open
14_chapter 4.pdf5.95 MBAdobe PDFView/Open
15_chapter 5.pdf67.04 kBAdobe PDFView/Open
16_references.pdf223.16 kBAdobe PDFView/Open

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