Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/15789
Title: Biodegradation of Anionic surfactant Sodium Dodecyl Sulphate (SDS) and analysis of its metabolic products
Researcher: Ambily, P S
Guide(s): Jisha, M S
Keywords: Biosciences
Microbiology
Anionic surfactant Sodium Dodecyl Sulphat
Upload Date: 11-Feb-2014
University: Mahatma Gandhi University
Completed Date: August 2010
Abstract: ABSTRACT The anionic surfactant Sodium dodecyl sulphate (SDS), the core component of detergent and cosmetic product formulations, contribute significantly to the pollution profile of sewage and wastewaters of all kinds. Due to its high foaming capabilities which can cause numerous problems in sewage treatment facilities as well as the direct toxic effects on many different organisms in the ecosystem, it is generally considered to be a serious pollutant. In this study, 44 SDS degrading strains were isolated by soil enrichment methods and the utilization efficiency was assessed by methylene Blue Active Substances (MBAS) assay and HPLC .The most efficient SDS degrading isolate was selected and identified as Pseudomonas aeruginosa MTCC 10311 based on phenotypic features and 16 S rDNA typing (HM 214777).The selected isolate harbor plasmid but plasmid is not completely responsible for SDS degrading trait. Different physiological characters such as PH, substrate concentration, temperature, incubation time, aeration, carbon sources and nitrogen sources were optimized for efficient SDS degradation. Attempts were made to study the metabolic byproducts of SDS degradation using FTIR and GC-MS analysis. Analysis of ether extracts of surfactant established the sequential production of dodecanol, dodecanal and decanoic acid. At this point, the pathway diverged into the formation of acid residues through beta oxidation. The enzyme responsible for SDS degradation was extracted and purified. The enzyme activity was only 0.0546 U/mg in the crude extract, which on purification by streptomycin sulphate, dialysis, DEAE cellulose and sephadex G200 gave enzyme units of 3.04 U/mg .The partially purified enzyme could give a maximum SDS degradation of 75% at 4 % of enzyme concentration. newlineThe selected organism was used for the treatment of synthetic wastewater containing SDS. The reduction of SDS and COD in the synthetic wastewater by free cells and immobilized cells of the isolate Pseudomonas aeruginosa were investigated.
Pagination: 160p.
URI: http://hdl.handle.net/10603/15789
Appears in Departments:Faculty of Science

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01_title.pdfAttached File76.34 kBAdobe PDFView/Open
02_declaration.pdf68.62 kBAdobe PDFView/Open
03_certificates.pdf135.85 kBAdobe PDFView/Open
04_acknowledgements.pdf55.37 kBAdobe PDFView/Open
05_abstract.pdf137.7 kBAdobe PDFView/Open
06_preface.pdf212.38 kBAdobe PDFView/Open
07_abbreviations.pdf104.25 kBAdobe PDFView/Open
08_contents.pdf147.62 kBAdobe PDFView/Open
09_list of tables.pdf14.7 kBAdobe PDFView/Open
10_list of figures.pdf22.38 kBAdobe PDFView/Open
11_list of plates.pdf61.33 kBAdobe PDFView/Open
12_chapter 1.pdf746.44 kBAdobe PDFView/Open
13_chapter 2.pdf756.63 kBAdobe PDFView/Open
14_chapter 3.pdf1.12 MBAdobe PDFView/Open
15_chapter 4.pdf611.01 kBAdobe PDFView/Open
16_chapter 5.pdf403.69 kBAdobe PDFView/Open
17_references.pdf412.3 kBAdobe PDFView/Open
18_appendix.pdf164.91 kBAdobe PDFView/Open


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