Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/15502
Title: The integrated analysis of genome protein interaction and metabolic pathway to improve lovastatin biosynthesis in Aspergillus Terreus NIH2624
Researcher: Subazini T K
Guide(s): Krishnan, C N
Keywords: Aspergillus Terreus, cancer, renal diseases, biochemical pathways
Upload Date: 3-Feb-2014
University: Anna University
Completed Date: 
Abstract: Lovastatin, the first statin drug approved by the FDA in 1987, is a potent drug used in the treatment of cardiovascular disease by reducing the levels of low-density lipoproteins (LDL), the so-called bad cholesterol. It also plays an important role in the treatment of Alzheimerand#8223;s disease, cancer and renal diseases. The study of biochemical pathways in A. terreus will provide knowledge on byproduct reduction and redirecting the flux flow through improved lovastatin production. Aspergillus terreus ATCC 20542 is widely used in industry for the production of lovastatin, but the whole genome sequence is not fully analyzed. So the computational analysis of pathways and interaction networks was done for A. terreus strain NIH2624 whose whole genome sequence is publicly available. The clues obtained from the sequence analysis of strain NIH2624 can be utilized to increase the yield of lovastatin. The protein interaction network analysis was done to determine the interacting partners of lovastatin biosynthetic cluster proteins and validation. The validation of the clues obtained from the above analysis was confirmed in the wet laboratory, and the results obtained were in accordance with the computational result. Sodium pyruvate, used as an inhibitor of isocitrate lyase increased the production of lovastatin from 0.95and#956;g/ml to 9.68and#956;g/ml. Calcium chloride, an inhibitor of 6-methyl salicylic acid synthase (precursor of patulin) increased the production of lovastatin from 0.95and#956;g/ml to 5.8and#956;g/ml. Sodium oxalate, an inhibitor of lactate dehydrogenase reduced the production of lovastatin from 0.95and#956;g/ml to 0.67and#956;g/ml. newline newline newline
Pagination: xvi, 135
URI: http://hdl.handle.net/10603/15502
Appears in Departments:Faculty of Technology

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01_title.pdfAttached File44.23 kBAdobe PDFView/Open
02_certificates.pdf170.21 kBAdobe PDFView/Open
03_abstract.pdf46.44 kBAdobe PDFView/Open
04_acknowledgement.pdf28.52 kBAdobe PDFView/Open
05_contents.pdf59.86 kBAdobe PDFView/Open
06_chapter 1.pdf212.45 kBAdobe PDFView/Open
07_chapter 2.pdf94.43 kBAdobe PDFView/Open
08_chapter 3.pdf117.11 kBAdobe PDFView/Open
09_chapter 4.pdf181.73 kBAdobe PDFView/Open
10_chapter 5.pdf66.39 kBAdobe PDFView/Open
11_chapter 6.pdf324.56 kBAdobe PDFView/Open
12_chapter 7.pdf1.04 MBAdobe PDFView/Open
13_appendices 1 to 6.pdf220.26 kBAdobe PDFView/Open
14_references.pdf157.37 kBAdobe PDFView/Open
15_publications.pdf61.28 kBAdobe PDFView/Open
16_vitae.pdf48.92 kBAdobe PDFView/Open


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