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Title: Studies on electrospun eri silk fibroin scaffold for biomedical applications
Researcher: Muthumanickkam A
Guide(s): Subramanian, S.
Keywords: Electrospun, Eri Silk, Thermo-gravimetric analyser, Differential scanning calorimeter, X-ray diffractormeter, Fourier transorm infrared spectroscope, scanning electron microscopy
Upload Date: 20-Jan-2014
University: Anna University
Completed Date: 
Abstract: Mulberry (Bombyx mori) silk is used for biomedical applications in different form such as film, foam, sponge, hydrogel and electrospun mat. Sericin content in the silk is one of the factors which induce an inflammatory response in the tissue. Eri silk (Samia cynthia ricini) contains low amount of sericin than mulberry and other wild silks. In the present work, a comparison was made between the scaffolds prepared by the electrospinning method from mulberry and eri silk fibroins. The majority of the fibres obtained from both the silk fibroins were in the diameter range of 401 to 500 nm. Since the silk fibroin scaffold had the problem of curling and shrinking, when treated with the solutions used for tissue culture, they were treated with ethanol to improve its structural stability and crystallinity. The physical and chemical properties of the scaffolds were assessed using Thermo-gravimetric analyser (TGA), Differential scanning calorimeter (DSC), Fourier transform infrared spectroscope (FTIR) and X-ray diffractometer (XRD). In order to study biodegradability, the electrospun fibrous mat was degraded in vitro in protease enzyme. The weight loss obtained after 30 days of degradation was found to be 34%. The in vitro enzymatic degradation of the fibrous mat was confirmed through Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy. The eri silk fibroin scaffold was tested for tensile strength, before and after 15 day s degradation. In the present work, a composite of hydroxyapatite (Hap) and the eri silk fibroin (ESF) scaffold was prepared, by alternately soaking the ESF scaffold in a solution of calcium chloride and sodium diammonium phosphate. The scanning electron microscopy (SEM) and fluorescent microscopy showed the impressive cell growth and favourable morphological features of the scaffolds. The study revealed that the ESF- Hap scaffold is better suited for MSC cell growth than the pure ESF scaffold. newline newline newline
Pagination: xxiv, 184
Appears in Departments:Faculty of Technology

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01_title.pdfAttached File34.28 kBAdobe PDFView/Open
02_certificates.pdf1.61 MBAdobe PDFView/Open
03_abstract.pdf26.78 kBAdobe PDFView/Open
04_acknowledgement.pdf17.57 kBAdobe PDFView/Open
05_contents.pdf58.98 kBAdobe PDFView/Open
06_chapter 1.pdf29.76 kBAdobe PDFView/Open
07_chapter 2.pdf601.34 kBAdobe PDFView/Open
08_chapter 3.pdf2.41 MBAdobe PDFView/Open
09_chapter 4.pdf2.54 MBAdobe PDFView/Open
10_chapter 5.pdf906.1 kBAdobe PDFView/Open
11_chapter 6.pdf2.31 MBAdobe PDFView/Open
12_chapter 7.pdf49.89 kBAdobe PDFView/Open
13_references.pdf96.91 kBAdobe PDFView/Open
14_publications.pdf14.77 kBAdobe PDFView/Open
15_vitae.pdf13.67 kBAdobe PDFView/Open

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