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Title: In vivo Transcriptome Analysis of Plasmodium falciparum Clinical Isolates Glimpses into Molecular Events in Complicated Malaria
Keywords: quotClinical Isolates, Malariaquot
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Malaria in humans is caused by six species of Plasmodium, of which Plasmodium falciparum is considered the most virulent and responsible for much of the mortality. The challenges in combating malaria can largely be attributed to the complex life cycle of this parasite coupled with its inherent ability to adapt to its host environment. Additional parasite traits that may contribute to its pathogenesis are virulence factors which interact directly with the host, use of multiple host cell invasion pathways, remodeling of host cell for survival and the ability to transmit from one host to another. Importantly, most of the aforementioned factors are under newlinenatural selection and may evolve in response to the changing host s genetic and physiological environment. Any phenotypic variation or adaptive changes of malaria parasites in response to changing environment of the host may be reflected through the differential regulation of gene newlineexpression. Although, gene expression studies have been carried out on this parasite and has given an extraordinary amount of information about the parasite biology, our understanding about the transcriptome of this parasite in vivo still remains elusive. Pathogenesis studies in other systems have clearly demonstrated that organisms have distinct biology in vivo comparison to in vitro. Studies which have tried to partially mimic the host newlineconditions in culture showed subsets of genes differentially regulated in response to these changes, suggesting that the host provides a substantially different environment compared to laboratory conditions with a distinct effect on regulation and expression of parasite genes. In temperate and sub-tropical regions of Asia and Latin America, complicated malaria manifests as hepatic dysfunction or renal dysfunction and is seen in all age groups. There has been a concerted focus on understanding the patho-physiological and molecular basis of complicated malaria in children, much less is known about it in adults. Central to this thesis is dissecting the in vivo
Pagination: 14.2 MB
Appears in Departments:Biological Science

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