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Title: Molecular mechanism of anticancer activity of phycocyanin in triple negative breast cancer cells
Keywords: Anti-neoplastic
MDA MB 231 cells
University: Sri Ramachandra University
Completed Date: 14/12/2016
Abstract: Triple negative breast cancers represent an important clinical challenge as these cancers do not respond to conventional endocrine therapies or other available targeted agents. Phycocyanin PC a natural water soluble and non toxic molecule is shown to have potent anti cancer property In this study we determined the efficacy of PC as an anti-neoplastic agent in vitro on a series of breast cancer cell lines We studied effects of PC in inducing DNA damage and apoptosis through western blot and qPCR Also, antimetastatic and anti angiogenic properties were studied by classic wound healing and vasculogenic mimicry assays We found that triple negative MDA MB 231 cells were most sensitive to PC as compared to other cells They also showed decreased cell proliferation and reduced colony formation ability upon treatment with PC. Profile of Cell cycle analysis showed that PC caused G1 arrest which could be attributed to decreased mRNA levels of Cyclin E and CDK2 and increased p21 levels. Mechanistic studies revealed that PC induced apoptosis as evident by increase in percentage of annexin positive cells increase in H2AX levels and by changing the Bcl2 Bax ratio followed by release of cytochrome C and increased Caspase 9 levels MDA MB 231 cells treated with PC resulted in decreased cell migration and increased cell adhesive property and also showed anti angiogenic effects We also observed that PC suppressed cyclooxygenase 2 expression and prostaglandin E2 production All these biological effects of phycocyanin on MDA MB 231 cells could be attributed to decreased MAPK signaling pathway We also observed that PC is non toxic to non-malignant cells platelets and RBCs Taken together, these findings demonstrate for the first time, that PC may be a promising anti neoplastic agent for treatment of triple negative breast cancers newline
Pagination: 1-233
Appears in Departments:College of Biomedical Sciences

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01_title.pdfAttached File15.4 kBAdobe PDFView/Open
02_certificate.pdf61.94 kBAdobe PDFView/Open
03_declaration.pdf139.91 kBAdobe PDFView/Open
04_table of contents.pdf185.04 kBAdobe PDFView/Open
05_list of figures.pdf553.5 kBAdobe PDFView/Open
06_list of tables.pdf38.3 kBAdobe PDFView/Open
07_acknoledgements.pdf73.41 kBAdobe PDFView/Open
08_chapter 1.pdf1.26 MBAdobe PDFView/Open
09_chapter 2.pdf294.03 kBAdobe PDFView/Open
10_chapter 3.pdf1.63 MBAdobe PDFView/Open
11_chapter 4.pdf1.03 MBAdobe PDFView/Open
12_chapter 5.pdf667.93 kBAdobe PDFView/Open
13_chapter 6.pdf722.56 kBAdobe PDFView/Open
14_chapter 7.pdf696.07 kBAdobe PDFView/Open
15_chapter 8.pdf1.71 MBAdobe PDFView/Open
16_conclusion.pdf273.84 kBAdobe PDFView/Open
17_references.pdf280.44 kBAdobe PDFView/Open
18_appendix.pdf47.69 kBAdobe PDFView/Open
19_publication.pdf3.16 MBAdobe PDFView/Open

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