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Title: Investigation of Microsporidial Keratitis in India A Clinical Microbiological Molecular and Proteome Approach
Keywords: Microsporidial Keratitis, Clinical Microbiological Molecular, Proteome
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Microsporidia, an obligate intracellular parasite, has been recently recognized as a causative agent of infective keratitis. There is an under reporting of this entity owing to the diagnostic difficulties involved and comprehensive studies addressing various aspects such as the etiology, diagnosis and pathogenesis of MK are lacking from India. The purpose of this study was to describe the clinico-microbiological features and protein profile of microsporidial species causing keratitis. Retrospective review of laboratory proven cases seen at the L.V.Prasad Eye Institute between January 2002 and June 2006 showed 41 of 7820 patients with suspected microbial keratitis (0.5%) to be diagnosed with microsporidial keratitis. Diffuse, multifocal, punctate, epithelial corneal lesions with conjunctivitis were noted in 32; all responded to medical treatment. Six of nine patients with deep stromal keratitis required penetrating keratoplasty and 3/9 were diagnosed by histopathology. Evaluation of conventional microbiological and histopathological techniques revealed that potassium hydroxide with calcofluor white and modified Ziehl Neelsen (1% H2SO4) stains are ideal for diagnosis of microsporidial keratitis on corneal scrapings. Gram s chromotrope and modified Ziehl Neelsen (1% H2SO4) stains are dependable and cost effective methods of diagnosing microsporidiosis in ocular tissues. newline newlineTo determine the most suitable in vitro system for culture of microsporidia, varying concentrations (1x104 1 x 108 spores / ml) of Vittaforma corneae, Encephalitozoon hellem, Encephalitozoon cuniculi and Encephalitozoon intestinalis were inoculated in vero, SIRC and HeLa cell lines and monitored over time. The minimum infective dose was the least for vero ( 1 x 104 spores/ml ), compared to SIRC (1 x105 spores/ml) and HeLa ( 1 x 107 spores/ml) cell lines. Greater susceptibility of vero cell line supports its inclusion in culture protocols for the growth of microsporidial. However, when validated on clinical samples, it was less sensitive.
Pagination: 51.8 MB
Appears in Departments:Biological Science

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