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Title: quotStudiies on Sequence Diiversiity and Characteriizatiion of the Apiicall Membrane Antiigen of Pllasmodiium iin Indiian Isollatesquot
Researcher: VIDYA RAJESH
Guide(s): Ashiis K.. Das
Keywords: Plasmodium, Malaria, hepatocytes, Erythrocyte Invasion
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Malaria parasites exhibit sequence diversity for a number of stage specific antigens. The newlineApical Membrane Antigen (AMA-1) is one of the important candidate vaccine antigens, newlineexpressed in the sporozoite and merozoite stage of infection. This protein is presumed to newlineplay a important role in invasion of host cells. Several Studies have proved that Apical newlineMembrane Antigen (AMA-1) is an effective target eliciting a protective immune newlineresponse. The sequence variation was investigated for both Plasmodium falciparum and newlinePlasmodium vivax Apical Membrane antigen from different Indian isolates in this study. newlineOur study revealed a much higher degree of sequence diversity than reported so far for newlineboth Pf AMA-1 as well as Pv AMA-1. Moreover, the presence of 5 novel haplotypes was newlinealso identified for the P.vivax AMA-1 among the Indian isolates studied. Hence, this newlinestudy is significant in indicating the antigenic repertoire of isolates in a malaria endemic newlinecountry like India. newlineVariant alleles were cloned and expression of the full length AMA-1 of P. falciparum newlinewas tried for immunological characterization. However, the expression of full length newlineAMA-1 protein was not successful and hence synthetic peptides were designed against newlinethe variants of one already reported B cell epitope in the ectodomain region of AMA-1. newlineThe antibody response to the peptides delineating linear epitopes from five different newlinevariants of an epitope of AMA - 1 of P.falciparum was studied in naturally infected newlinepopulation from Rajasthan, India. Analysis of the antibody profiles of these 25 newlineP. falciparum infected patients was also carried out. newline
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Appears in Departments:Biological Science

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