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Title: Design and Development of Confocal Microscopic Techniques and Studies on Passive Permeability of Nuclear Membranes
Guide(s): P. Nandakumar
Keywords: Confocal Microscopic Techniques, Passive Permeability, Nuclear Membranes
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Studies on biomolecular transport through nuclear membranes are of interest from a fundamental newlinephysics perspective as well as from the point of view of biological applications. The nuclear pore newlinecomplexes (NPCs) in the nuclear membrane are the only known passage way for newlinemacromolecules to enter into and exit from the nucleus. The NPCs prevent any foreign DNA newlinefrom entering the nucleus and regulate the transport of desired molecules in an organized and newlineorderly fashion. Though it is well known that they play a central role in the regulation of cell newlinefunctions, there is still no clear agreement on the structure and pore size of the NPCs. Moreover newlinethe mechanisms involved and the environment dependent role of the NPCs in the regulation of newlinenuclear transport is not yet thoroughly probed. A proper understanding of the transport newlinephenomena will help in developing methodologies which will be of interest to biomedical newlineresearch, especially related to gene therapy. newlineOptical microscopic techniques play an important role in biomedical research. In this newlineresearch work we first developed a confocal laser scanning microscope (CLSM) for studying newlinebiomolecular transport through nuclear membranes. Even though confocal fluorescence newlinemicroscopes are commercially available, most of them are not flexible to be used in laboratory newlineenvironment where different types of experiments are to be carried out. When compared to newlinecommercial microscopes, the CLSM designed in the laboratory is cost effective and versatile, newlineand the same microscope can be used in different geometries such as photothermal microscopy newlineand nonlinear microscopy. An inverted microscope, X-Y galvanometric mirror scanner, data newlineacquisition card (DAQ), an Argon ion laser or HeNe laser and optics available in the laboratory newlineare used in the construction of CLSM. Reflectance or fluorescence signal is coupled to a photo newlinedetector through an optical fiber which acts as the confocal aperture. For scanning, data newlineacquisition and image reconstruction, we use a DAQ card and a home written LabVIEW newlineprog
Pagination: 7.78 MB
Appears in Departments:Biological Science

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