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Title: Characterization and Analysis of Plastid DNA of the Apicomplexan Parasite Plasmodium vivax from India
Guide(s): Ashis K. Das
Keywords: Plastid DNA, Apicomplexan Parasite Plasmodium vivax
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Apicomplexan parasite Plasmodium is characterized by a three four membrane organelle called Apicoplast. The organelle is considered indispensable for the survival of the parasite as it is the site for various biochemical and metabolic pathways, such as, Type II fatty acid synthesis pathway and non mevalonate pathway for isoprenoid biosynthesis. Various enzymatic steps within these pathways make the organelle a putative drug target. The organelle also contains a circular DNA (plastid DNA) having its own origin of replication and found in multiple copies. A complete 35kb plastid DNA has been reported from human malaria parasite Plasmodium falciparum. The genome contains many conserved regions and ORFs. The major regions present are large and small subunit rRNA genes, sufB (ORF470) gene, clpC (casienolytic protease) gene, tufA (elongation factor Tu) gene and various other ribosomal protein genes. Besides this, 7 ORFs of varying sizes and unknown functions are also present in the genome. Inspite of the importance of Apicoplast in the parasite, the organelle and its genome has not been detailed (except for partial sequence of few genes) as yet from another major human malaria parasite Plasmodium vivax. Recent reports briefing the severe manifestations related to P. vivax malaria make the study of Apicoplast in this parasite a necessity. Thus, our study aims to characterize major genes from the plastid genome of Plasmodium vivax from Indian isolates. newline newlineIn this study we have amplified the major genes of P. vivax Apicoplast from the Indian field isolates and have studied the variations present in the Apicoplast genome of P. vivax as compared to that of P. falciparum. A comparative analysis of various plastid genes from P. vivax and P. falciparum reveals approximately 5 10% variations. Sequence and structural analysis of respective proteins do not show any variations in the active sites. With the help of designed peptides based upon plastid protein sequences, antibodies were raised and localization studies.
Pagination: 6.84 MB
Appears in Departments:Pharmacy

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