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Title: Studies on Preparation Characterization and Pharmacokinetics of Etoposide Loaded Nanoparticles
Guide(s): Ranendra N. Saha
Keywords: Pharmacokinetics of Etoposide Loaded Nanoparticles
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Present research aims at design and development of nanoparticulate delivery system, newlinefor better delivery of etoposide for extended period of time for better therapeutic efficacy. newlineMain objective of the present work was to prepare and characterize etoposide loaded newlinenanoparticles using different biodegradable and/or bioacceptable polymers alone and in newlinecombination and their characterization and in vivo study of their pharmacokinetic and newlinedistribution in animal model. newlineAs a part of this study, suitable analytical methods were developed for the newlineestimation of etoposide in formulations and different study samples. According to the need, newlineUV spectrophotometric, spectrofluorimetric and HPLC method with UV detection were newlinedeveloped and validated. These methods were sensitive, accurate, precise and found to be newlinesuitable for analysis of etoposide in formulations and study samples. Under preformulation newlinestudies, pH - solubility, pH - stability, partition coefficient and drug-excipient interaction newlinestudies at different storage conditions were carried out for etoposide. These studies newlineindicated the poor aqueous solubility and degradation of etoposide at extreme acidic and newlinealkaline conditions. newlineNanoparticle formulations encapsulating etoposide were prepared using newlinenanoprecipitation method or solvent evaporation method depending on the polymer used. newlineBiodegradable and biocompatible polymers, poly lactic glycolic acid co polymers (PLGA newline50/50, PLGA 75/25 and PLGA 85/15), poly-e-caprolactone and Eudragit┬« L 100 were newlineselected for preparation. Formulations were optimized by changing various process newlineparameters during preparation. Concentration of stabilizers (Pluronic F 68 and Poly vinyl newlinealcohol), amount of polymer and etoposide, volume of aqueous phase and organic phase newlineused for preparation of nanoparticles were optimized. Morphology of the prepared newlinenanoparticles was studied by transmission electron microscopic and atomic force newlinemicroscopic techniques. Size distribution, polydispersity index and zeta potential for the newlineprepared nanoparticles.
Pagination: 27 MB
Appears in Departments:Pharmacy

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