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Title: Investigations of anticancer activity of ethanol extract fractions of Cardiospermum halicacabum Linn and Combretum albidum G Don
Researcher: Zalke, Ashish Suresh
Guide(s): Duraiswamy, B
Keywords: anticancer drugs, C. halicacabum, C. albidum, cytotoxicity, apoptosis studies
University: JSS University
Completed Date: 19/05/2014
Abstract: The ethanol extracts of C. halicacabum and C. albidum have been subjected for their in vitro and in vivo antioxidant and anticancer activity using standard procedures. Aqueous and ethanol extracts fractions also subjected for in vitro cytotoxicity by MTT, SRB assay using normal and cancerous cell lines and trypan blue dye exclusion method using DLA cells and apoptosis studies. Aqueous and ethanol extracts from the aerial parts of the C. halicacabum, leaves androots of the C. albidum have been prepared. Preliminary phytochemical analysis of the extracts found to contain glycosides, phytosterols, saponins, flavonoids, carbohydrates, and triterpenoids. Aqueous and ethanol extracts of the aerial parts of C. halicacabum and leaves and roots of the C. albidum have been subjected for in vitro cytotoxicity by MTT, SRB assay using normal and cancerous cell lines and trypan blue dye exclusion method using DLA cells. The ethanol extract of roots of the C. albidum has been found to have good amount of phenols and flavonoids. In vivo anticancer study has been carried out by DLA cell model and solid tumour model. In the in vivo anticancer study by DLA cell model, the mean survival time, percentage ILS, percentage change in body weight, hematological, biochemical and antioxidant parameters have been evaluated. Apoptosis activity of isolated fractions has been carried out by fluorescence microscopy, DNA laddering technique and cell cycle analysis by flow cytometry. Results have shown that the isolated fractions of both the plants have been found to induce apoptosis in cancerous cell lines. Thus the present study supports the ethnobotanical claim of C. halicacabum for the treatment of cancer. The leaves and roots of C. albidum prove that they have dose dependent anticancer activity.
Pagination: i-xii, p.194
Appears in Departments:College of Pharmacy

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01_title.pdfAttached File136.95 kBAdobe PDFView/Open
02_certificates.pdf287.39 kBAdobe PDFView/Open
03_acknowledgement.pdf129.05 kBAdobe PDFView/Open
04_contents.pdf21.81 kBAdobe PDFView/Open
05_list of tables figures.pdf144.21 kBAdobe PDFView/Open
06_chapter 1.pdf674.24 kBAdobe PDFView/Open
07_chapter 2.pdf200.61 kBAdobe PDFView/Open
08_chapter 3.pdf862.23 kBAdobe PDFView/Open
09_chapter 4.pdf276.74 kBAdobe PDFView/Open
10_chapter 5.pdf617.34 kBAdobe PDFView/Open
11_chapter 6.pdf8.39 MBAdobe PDFView/Open
12_chapter 7.pdf514.16 kBAdobe PDFView/Open
13_chapter 8.pdf264.77 kBAdobe PDFView/Open
14_references.pdf549.36 kBAdobe PDFView/Open
15_appendix.pdf1.66 MBAdobe PDFView/Open

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