Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/11088
Title: Identification and Characterization of a Calcium Oxalate Crystal Growth Protein Inhibitor from Human Renal Stone Matrix
Researcher: Priyadarshini
Guide(s): Tandon, Chanderdeep
Keywords: Calcium Oxalate
Stone Crystals
Upload Date: 11-Sep-2013
University: Jaypee University of Information Technology, Solan
Completed Date: 17/03/2010
Abstract: Kidney stone formation is a complex process involving multiple factors. Although saturation product for CaOx is frequently exceeded in normal urine, most humans do not form stones. It has been assumed that inhibitors of urolithiasis have protective effect, although their precise role has not been defined. Kidney stones invariably comprise a combination of inorganic crystals and organic macromolecules consisting principally of proteins. Many proteins occur in stones, but their role in urolithiasis remains unknown. Calculi contain some proteins normally present in urine, in addition to others arising from injury inflicted by the stones themselves, making it impossible to discriminate between those that bind to the stone as it grows, but play no role in its development, and those that may be involved in regulating the formation of stone crystals. The inhibition is generally understood to arise mainly from the non-dialyzable molecules of urine, particularly acidic glycoproteins and glycosaminoglycans. Some inhibitor molecules have been identified, including Tamm Horsfall protein, uropontin , calgranulin, bikunin, and prothrombin F1 fragment. There are several hypotheses regarding kidney stone formation. According to one hypothesis, it is related to intratubular crystal nucleation, growth, and aggregation. While other hypothesis explains that the locale of crystal deposition is at a renal interstitium near or at the tip of renal papillae. newlineInsoluble nature of kidney stone poses many challenges in extraction of proteins present in its organic matrix; therefore, a comparison of different methods of extraction from human calcium oxalate containing stones was done to examine the soluble matrix proteins involved in their biomineralization process. It was observed that EGTA extract exhibited highest inhibitory activity (98%) towards CaOx crystal growth followed by acetic acid (6.47%) and SDS extract (2.64%). Therefore, EGTA extraction method was selected for the renal stone extraction to carry out the further studies.
Pagination: 
URI: http://hdl.handle.net/10603/11088
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File82.48 kBAdobe PDFView/Open
02_acknowledgement.pdf182.12 kBAdobe PDFView/Open
03_contents.pdf123.39 kBAdobe PDFView/Open
04_list of tables figures.pdf248.29 kBAdobe PDFView/Open
05_chapter 1.pdf433.08 kBAdobe PDFView/Open
06_chapter 2.pdf5.25 MBAdobe PDFView/Open
07_chapter 3.pdf1.69 MBAdobe PDFView/Open
08_chapter 4.pdf2.64 MBAdobe PDFView/Open
09_chapter 5.pdf1.43 MBAdobe PDFView/Open
10_conclusion.pdf369.56 kBAdobe PDFView/Open
11_list of publications.pdf176.85 kBAdobe PDFView/Open
12_references.pdf3.53 MBAdobe PDFView/Open


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