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Researcher: Bandana
Guide(s): Sharma, Neelam
Tea husbandary
Upload Date: 19-Aug-2013
University: Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya
Completed Date: 2010
Abstract: newlineABSTRACT newline A field experiment was conducted during the year 2008 and 2009 to study the effect of glyphosate on biochemical attributes of tea and its degradation behaviour in soil and tea. Experiment was laid out in randomized block design (RBD) consisted of four treatments viz. glyphosate 2.0 kg ha-1, glyphosate 1.0 kg ha-1, glyphosate 0.5 kg ha-1 and control. Tea samples were collected from the laid out experiment at fortnightly interval from imposed treatments for biochemical and degradation studies and soil samples were also collected at same time intervals for degradation studies. Glyphosate at three levels of application i.e. 0.5 kg ha-1, 1.0 kg ha-1 and 2.0 kg ha-1 decreased the values of all biochemical constituents i.e. total amino acids, amino acid profile, comprising six groups viz. aspartic acid, methionine, phenylalanine, leucine, theanine and glutamic acid, polyphenols, catechins, polyphenol oxidase activity in tea leaves at zero day after application except shikimic acid content in tea leaves. The values of shikimic acid in tea leaves increased significantly on glyphosate application. In general, all biochemical constituents were influenced significantly upto 15 days after herbicide application except shikimic acid and polyphenol oxidase activity in tea leaves which were affected significantly upto 30 days after herbicide application. However, thereafter no significant effect was observed in any of biochemical constituents under study during both years. The herbicide dose dependent inverse relationship was noticed for all biochemical constituents except shikimic acid in tea leaves. The degradation data generated in the present investigation during both years indicated that glyphosate at three rates of application i.e. 0.5 kg ha-1, 1.0 kg ha-1 and 2.0 kg ha-1 persisted in soil upto 30, 45 and 60 days, respectively. More than 85% of applied glyphosate in soil dissipated within 45 days of herbicide application. Whereas in tea leaves, the residue were detectable only upto 15 days in all three glyphosate treatments. Thus, revealing that degradation of glyphosate was faster in plant as compared to soil. The logarithmic plot of herbicide versus time fitted first order kinetics decay curves during both years. Half life values for glyphosate at three doses during both years varied in range 5.80 to 19.10 days in soil and 5.82 to 7.91 days in tea. Since, glyphosate concentrations in tea leaves even at zero day sampling were well below maximum residue limit (MRL) for glyphosate in tea (0.5 mg kg-1) set by WHO/FAO(, therefore it can be concluded from the present study that to use glyphosate in tea plantations at three application rates i.e. 0.5 kg ha-1, 1.0 kg ha-1 and 2.0 kg ha-1 is safe both in terms of quality and food safety point of view. Further, it can also be inferred that glyphosate concentration was directly correlated with biochemical attributes in tea. newline
Pagination: 28.5cm.
Appears in Departments:Department of Chemistry and Biochemistry

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01_title.pdfAttached File164.46 kBAdobe PDFView/Open
02_certificate.pdf69.96 kBAdobe PDFView/Open
03_acknowledgement.pdf44.72 kBAdobe PDFView/Open
04_list of contents.pdf9.23 kBAdobe PDFView/Open
05_list of abbreviations, tables, plates and fig. etc..pdf51.47 kBAdobe PDFView/Open
06_chapter-1.pdf184.9 kBAdobe PDFView/Open
07_chapter-ii.pdf81.94 kBAdobe PDFView/Open
08_chapter-iii.pdf358.89 kBAdobe PDFView/Open
09_chapter-iv.pdf2.04 MBAdobe PDFView/Open
10_chapter-v.pdf105.5 kBAdobe PDFView/Open
11_chapter-vi.pdf64.45 kBAdobe PDFView/Open
12_literature cited.pdf98.5 kBAdobe PDFView/Open
13_appendix.pdf52.1 kBAdobe PDFView/Open

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