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Title: A Study on the Therapeutic Potential of Silver and Gold Nanobioconjugates from Clitoria ternatea Leaves
Researcher: Sudha Devi, M
Guide(s): Padma, P R
Keywords: Silver Nanobioconjugates
Gold Nanobioconjugates
Clitoria ternatea
methanolic leaf extracts
in vitroand in vivo anti-inflammation
University: Avinashilingam Deemed University For Women
Completed Date: 25.02.2016
Abstract: Objectives: The green synthesis of silver and gold nanobioconjugates from the leaf extracts of newlineClitoria ternatea bearing blue and white flowers and their bioactivities. newlineMethodology: The leaves, seeds and roots of the two varieties of Clitoria ternatea were newlineexamined for their antioxidant, antibacterial activities after extraction with solvents of varying newlinepolarity. The methanolic leaf extracts of both varieties were selected for further analysis. newlineNanobioconjugates were prepared using silver and gold using sunlight, water bath heating and newlinemicrowave heating. As exposure to bright sunlight for 20 minutes gave the maximum yield,only newlinethis method was used further. The synthesized nanobioconjugates were then characterized. The newlineTEM and SEM images revealed that the size of the synthesized nanobioconjugates were well newlinewithin the range of nanoparticles with spherical shape. EDX, XRD, FTIR, zeta potential and PDI newlineshowed successful and stable conjugation of silver and gold with extract components, which newlinewere well dispersed. The antibacterial activity was assessed against clinical isolates. In vitro antiinflammatory newlineactivity was also evaluated using HRBC membrane stabilization, heat induced newlinehemolysis and proteinase inhibition activities. The results showed that the gold newlinenanobioconjugates exhibited better bioactivity than their silver counterpart. Biocompatibility of the newlinenanobioconjugates was determined in human blood cells using hemolysis, blood clotting time, newlinemorphological changes of RBC and blood lymphocytes. Drug release kinetics revealed that, all newlinethe four nanobioconjugates released their drug cargo steadily. All the experimental results proved newlinethat the synthesized nanobioconjugates were safe. Thein vivo anti-inflammatory activity was newlinetested using Swiss albino mice against both acute and chronic inflammations. The administration newlineof the silver and gold nanobioconjugates protected the mice against the both acute and chronic newlineinflammation than their unconjugated leaf extracts.
Pagination: 130 p.
Appears in Departments:Department of Biochemistry, Biotechnology and Bioinformatics

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02_certificate.pdf329.34 kBAdobe PDFView/Open
03_declaration.pdf237.06 kBAdobe PDFView/Open
04_acknowledgement.pdf93.69 kBAdobe PDFView/Open
05_ content.pdf75.72 kBAdobe PDFView/Open
06_ list of tables, figures & abbreviations.pdf58.24 kBAdobe PDFView/Open
07_abstract.pdf25.05 kBAdobe PDFView/Open
08_chapter1.pdf94.32 kBAdobe PDFView/Open
09_chapter2.pdf397.88 kBAdobe PDFView/Open
10_chapter3.pdf452.68 kBAdobe PDFView/Open
11_chapter4.pdf4.92 MBAdobe PDFView/Open
12_chapter5.pdf56.32 kBAdobe PDFView/Open
13_bibliography.pdf371.38 kBAdobe PDFView/Open

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