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Title: Ex vivo profiferation and characterization of adult limbal stem cells and their applications in treating ocular surface disorders caused by limbal stem cell deficiency
Researcher: Raheem, Anees Fatima Abdul
Guide(s): Vemuganti, Geeta Kashyap
Keywords: adult limbal stem cells, ocular surface disorders
University: Birla Institute of Technology and Science
Completed Date: 
Abstract: Ocular surface comprises of the ocular surface epithelia and the tear film. These epithelia include the corneal, limbal and conjunctival epithelium. Phenotypically the corneal epithelium is different from conjunctival epithelium. The corneal surface like any other mucosal surface is constituted by labile cells, which are regenerated every 6-7 days. This is facilitated by multiplication of stem cells located in limbus. In certain situations termed as limbal stem cell deficiencies (LSCD), the stem cells get damaged, thereby resulting in loss of transparency of cornea and visual disability. The treatment for this condition is limbal transplantation using auto or allograft limbal tissues. However for autologous limbal transplantation, the limitation is availability of adequate limbal tissue without compromising the donor surface. The allografting requires heavy immunosuppression. The ideal way would be expanding of the limbal stem cells ex-vivo and transplant them back into patient. This study proposes to carry out ex-vivo expansion of limbal stem cells using human amniotic membrane (HAM) as substrate. newlineThe protocol for ex-vivo culture of limbal epithelial cells was approved by the Institutional review board of LV Prasad Eye Institute. The explant culture technique of limbal epithelial cells over HAM was initially standardized by using cadaveric limbal tissue in a previous study. Following this 25 limbal tissues were collected from patients undergoing cataract surgery after obtaining the informed consent. These tissues were then cultured over the HAM using human corneal epithelium media supplemented with epidermal growth factor. All the 25 tissues showed good growth pattern. No contamination was seen in any of these 25 cultures. A check on contamination was kept by testing for Mycoplasma species in culture and other media components. This study is first in India wherein an attempt was made to culture limbal stem cells on HAM. Though the technique was based on techniques published previously, significant.
Appears in Departments:Pharmacy

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